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Quenching Enzyme

Title:Quenching Enzyme

Product description

Quenching enzyme - aquatic special compound enzymes

The harm of gram-negative bacteria to aquatic animals:

According to gram staining, bacteria can be divided into gram negative bacteria and gram positive bacteria. Among them, a lot of important pathogenic bacteria related to aquatic animal belongs to gram-negative bacteria. Gram-negative bacterial disease, as the most serious disease harming aquatic animals in China, which has a lot of pathogenic species and harm widely endemic areas aquatic breeding category, has caused great economic losses in aquaculture. The pathogen  which causes bacterial disease of aquatic animals has vibrio anguillarum, Vibrio parahaemolyticus,algin vibrio, Aeromonas punctata, Aeromonas hydrophila, Flavobacterium columnare,Edwardsiella tarda, etc, see table 1.

Tab. 1 Main gram-negative bacteria of aquatic pathogenic

Quorum sensing and gram-negative bacteria quenching enzymes:

Intestinal bacteria often produces and releases specific extracellular signal molecules in the gastrointestinal tract, signaling molecules accumulating to a certain concentration, can induce bacteria control bacteria molecular and cellular response, which is called bacterial quorum sensing. Quorum sensing plays an important role to maintain the normal function of bacteria, and regulate gene expression,as shown in figure 1. Quenching enzyme can degrade gram-negative bacterial quorum sensing signal molecules, destroy the quorumsensing, influence bacteria colonize, break biofilm formation, reduce the exotoxin, regulate gene expression, etc., as shown in figure 2. It also has the function of preventing bacterial disease of aquatic animals and improving aquatic animal health. By application of quenching in feed enzyme can reduce the use of feed additive such as antibiotics which is harmful to human body, it is important to obtain and guarantee high-quality and safe aquatic products.

Fig. 1 Bacterial quorum sensing

Fig.2 Signal molecular mechanisms of quenching enzyme degrading gram-negativebacteria

Product introduction

Quenching enzyme is high-efficient composite enzyme preparation expressed by molecular biology technology, and produced by the deep liquid fermentation, with five national invention patents and dozens of related thesis published in the domestic and foreign authoritative magazines. This product is rich in high-activity biological active substances, such as xylanase, β-mannase, probiotics, antitoxic factor and immune promoter, and so on. A large number of aquatic animal testing shows that quenching enzyme has the function of reducing the incidence of disease in aquaculture, improving economic efficiency of farming and safety of aquatic products,especially having special effects on main freshwater fish fulminant epidemic caused by Aeromonas hydrophila, such as tilapia canker disease, red-fin disease of eel, turtle bacterial sepsis,turtle bacterial sepsis, bullfrogs red legs disease. 

The characteristics and main functions

  • Improve the aquatic animal production performance: This product is rich in a variety of xylanase, glucose oxidase, antitoxic factor and immune promoter, reducing the colonization of Gram-negative bacteria, reducing toxins production and aquaculture diseases,enhancing the survival rate of aquatic animals and improving economic efficiency of breeding.
  • Promote aquatic animal intestinal health, improve immune function: Activity factors, such as xylanase, glucose oxidase, antitoxic factor and immune promoter, regulate Gram-negative bacteria gene expression, have no killing effect to G- bacteria (equivalent to add inactivated vaccine), do not affect the ecological structure of intestinal flora, contribute to promote animal intestinal health, strengthen aquaticanimal disease resistance.
  • Reduce antibiotic usage:Quenching enzyme can interfere with and break biofilm formation of G- bacteria, and thus reduce its engraftment in animal intestines, at the same time also strengthen the G- bacteria sensitivity to antibiotics; On the other hand quenching enzyme decrease G- bacteria toxin production, reduce bacteria pathogenicity and antibiotic use.

The stability of quenching enzyme

Enzymology properties of quenching enzyme:The effect of temperature and pH to the enzyme activity of quenching enzyme see chart 1. The relative enzyme activity of quenching enzyme in the range of pH from 6.0 to 8.5, remains at around 100%, so the quenching enzyme is suitable for the neutral gastrointestinal environment of aquatic animal; Quenching enzyme, derived from bacillus, has the very good heat resistance performance, more than 15 min of processing time at 70 ℃, 80 ℃, the relative enzyme activity keeps more than 90%, in the temperature of 90℃, the processing time is more than 3 min, relative enzyme activity keeps above 70%. As a result, quenching enzyme suits for aquatic animal gastrointestinal environment and process characteristics of aquatic feed processing.

Resistance to protease

Quenching enzyme has strong resistance to trypsin, chymotrypsin, hay bacillus coliprotease and collagenase. after processing for 1 h mixing with the neutral protease, the residual enzyme activity was more than 99.90%, after treatment for 1 h mixing with proteinase K, the residual enzyme activity was 50.19%. It can be demonstrated that quenching have resistance to a variety of protease, see below. 

Storage stability

Enzyme activity of quenching enzyme product keeps more than 95% storing for six months under the condition of room temperature, keeps more than 90% within 6 months inthe compound feed of aquatic product and keeps more than 80% in the premix. As aresult, quenching enzyme is of high stability in the feed and in room temperature for storage.

Method of application

Compound feed of aquatic animals:500 g/t; Concentrates or premix added in proportion.

Quenching enzyme mainly solves the following problems of aquaculture

(1)    Improve the aquatic animal disease resistance;

(2)    Improve the aquatic animal antibiotic sensitivity;

(3)    Reduce freshwater aquaculture diseases;

(4)    Improve the survival rate and increase the economic benefit of aquaculture.

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